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H.J. Kim
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P1.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 233)
- Event: WCLC 2015
- Type: Poster
- Track: Biology, Pathology, and Molecular Testing
- Presentations: 1
- Moderators:
- Coordinates: 9/07/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P1.04-111 - Establishing a Mouse Model for Radiation-Induced Esophagitis (ID 876)
09:30 - 09:30 | Author(s): H.J. Kim
- Abstract
Background:
To establish mouse models of radiation-induced esophagitis with fractionated irradiation using BALB/c or C57Bl/6 mice
Methods:
Thoracic irradiation at 0, 8, 12, or 15 Gy was given daily for 5 days by 320 kV X-ray irradiator to anesthetized, 6 week-old male BALB/c (n = 4~5 per group) or C57Bl/6 mice (n = 4 per group). Changes in the body weight and daily food intake were assessed for both strains of mice. At day 11, BALB/c esophagus was harvested and examined for the following assays: (i) histology by H&E staining; (ii) Cytokine array (R & D Systems); (iii) fluorescence-activated cell sorting (FACS) analysis by using Annexin V and propidium iodide (PI); (iv) quantitative real time-PCR (qRT-PCR) (Life Technologies) analysis.
Results:
We observed that fractionated irradiation produced a significant body weight reduction in Balb/c mice (20% by 12Gy X 5 and 30% by 15 Gy X 5). In contrast, C57Bl/6 mice seemed to be more resistant to fractionation irradiation as they exhibited little change in the body weight. As food intake in Balb/c mice was also significantly decreased at these doses compared to the control mice (p<0.05 for 12 Gy X 5 and P<0.01 for 15 Gy X 5), dose of 12Gy x 5 were selected for all assays. Histopathology of irradiated Balb/c mice showed erosive epithelium, mucosal detachment, and leukocyte infiltration. FACS analysis confirmed that irradiated esophagus had increased number of apoptotic cells, as evidenced by Annexin V and PI double positivity. We found that cytokines for C5/C5a, Timp-1 (tissue-inhibitor of metalloproteinases-1), Ccl2/Mcp-1 (monocyte chemoattractant protein-1), and Il-16 (interleukin-16) were increased in the irradiated esophagus compared to non-irradiated esophagus. qRT-PCR analyses revealed that Timp-1 as well as other genes involved in extracellular matrix remodeling including Pai-1 (plasminogen activator inhibitor-1), Gm-csf (granulocyte macrophage-colony stimulating factor), Vegf (vascular endothelial growth factor), and Sdf-1 (stromal-derived factor-1) were increased whereas Egf (epidermal growth factor), a potent mitogen for epithelial cells, was significantly decreased in the esophagus of irradiated mice.
Conclusion:
We established that BALB/c mice were more sensitive to fractionated irradiation than C57Bl/6 mice for developing symptoms reflecting radiation-induced esophagitis. In BALB/c mice, 12 Gy X 5 regimen seem to be the best schedule producing a significant reduction in the body weight and food intake, and histopathologic features similar to human esophagitis. Increased RNA transcripts for extracellular remodeling and cytokines indicate an active dynamics of tissue remodeling in the irradiated esophagus. Decreased Egf expression in the irradiated esophagus suggests that EGF may be a potential therapeutic strategy to treat radiation-induced esophagitis and we are currently investigating this strategy.
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P2.02 - Poster Session/ Treatment of Localized Disease – NSCLC (ID 210)
- Event: WCLC 2015
- Type: Poster
- Track: Treatment of Localized Disease - NSCLC
- Presentations: 1
- Moderators:
- Coordinates: 9/08/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P2.02-039 - Patterns-Of-Care Study of Stereotactic Ablative Radiotherapy for Lung Cancer in Korea (ID 164)
09:30 - 09:30 | Author(s): H.J. Kim
- Abstract
Background:
Stereotactic ablative radiotherapy (SABR) is an emerging effective technique for early stage lung cancer. We investigated the current practice patterns for stereotactic ablative radiotherapy (SABR) for lung cancer in Korea.
Methods:
A nationwide survey about experience with SABR for lung cancer was sent by e-mail to the radiation oncologists of 85 institutions in May 2014. SABR was defined as hypofractionated radiotherapy (1–8 fractions). The survey contained 23 questions, and those regarding technical details allowed multiple choices.
Results:
Of the 59 institutions that responded to the survey, 33 (56%) had used SABR for lung cancer. Thirty-seven radiation oncologists from these 33 institutions responded to the survey. Seventy-five percent of the oncologists had been treating lung cancer with SABR for less than 5 years, while 89% treat less than 20 cases annually. The most common planning method was rotational intensity-modulated technique (59%), followed by static intensity-modulated technique (49%). A wing board (54%) was most frequently used for immobilization, followed by the vacuum lock system (51%). Respiratory motion was managed by gating (54%) or abdominal compression (51%), and 86% of the planning scans were obtained with 4-dimensional computed tomography. More than half of the respondents (62%) treated daily if a multi-fraction regimen was used.
Conclusion:
The results of our survey indicated that SABR for lung cancer is being used increasingly in Korea, and that the majority of radiation oncologists using this therapy have limited experience in its use. There was wide variation among institutions with regard to the technical protocols, which indicates that standardization is necessary prior to the initiation of further nationwide multi-center, randomized studies.
