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Y. Sakata
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P1.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 233)
- Event: WCLC 2015
- Type: Poster
- Track: Biology, Pathology, and Molecular Testing
- Presentations: 1
- Moderators:
- Coordinates: 9/07/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P1.04-103 - Oncolytic Virus Therapy for Lung Cancers Using a Genetically Engineered Oncolytic Herpes Simplex Virus Type 1 G47Δ (ID 1076)
09:30 - 09:30 | Author(s): Y. Sakata
- Abstract
Background:
Lung cancer is the leading cause of cancer deaths in Japan and worldwide. Despite recent advances in targeted therapy, long-term survival of patients with lung cancer remains poor. Novel treatment approaches are needed to extend survival of these patients and improve control of this disease. Oncolytic virus therapy is a promising therapy for various tumor types. A third generation oncolytic herpes simplex virus type 1 (HSV-1), G47Δ, has been tested in clinical trials in Japan for glioma, prostate cancer, and olfactory neuroblastoma. In this study, we investigated the potential of G47∆ as a new therapeutic modality for human lung cancer.
Methods:
Human lung cancer cell lines A549 (adenocarcinoma), EBC-1 (squamous cell carcinoma), LU99 (large cell carcinoma) and SBC-3 (small cell carcinoma) were used. Infectivity and cytopathic effects of G47Δ on lung cancer cell lines were assayed in vitro. Viral replication was determined by standard viral plaque assay. For in vivo studies, athymic mice harboring established subcutaneous tumors and lung tumors generated with A549 or EBC-1 were used.
Results:
All cell lines were susceptible and sensitive to G47Δ irrespective of histological types. Viral replication assay resulted in approximately a 200-fold increase in virus titer by 48 h. In subcutaneous xenograft models, intraneoplastic inoculations with G47Δ significantly inhibited the tumor growth compared with those with mock. In orthotopic xenograft models, intrapleural inoculations with G47Δ prolonged the survival time.
Conclusion:
Oncolytic HSV-1 G47Δ was effective in human lung cancer cell lines. Direct intratumoral inoculation of G47Δ induced an obvious therapeutic effect on lung cancer, suggesting G47Δ may be a potent therapeutic modality for all histological types of lung cancer.
