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Z. Liu
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P1.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 233)
- Event: WCLC 2015
- Type: Poster
- Track: Biology, Pathology, and Molecular Testing
- Presentations: 1
- Moderators:
- Coordinates: 9/07/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P1.04-094 - NT5E(CD73) Is a Target of miR-30a-5p and Plays a Critical Role in Non-Small Cell Lung Cancer (ID 507)
09:30 - 09:30 | Author(s): Z. Liu
- Abstract
Background:
Background: CD73, is a glycosylphosphatidylinositol (GPI)-linked 70-kDa cell surface enzyme that catalyzes the dephosphorylation of extracellular AMP to adenosine, its dysregulation contributes to tumorigenesis and progression of a variety of malignancies, and it was suggested as a therapeutic target of cancers. But the functional relevance of CD73 and the mechanism underlying its dysregulation in lung tumorigenesis remained unclear. Here, we mainly focus on if CD73 has important functions in non-small-cell lung cancer (NSCLC) by: 1.evaluating the clinicopathologic significance of CD73 through analysing its expression in 38 human NSCLCs tissues using quantitative PCR, Western Blot; 2. determining its role in NSCLC using in vitro assays; 3. investigating the regulatory mechanism of CD73 dysregulation in NSCLS cell lines.
Methods:
Western blot analysis, real-time quantitative reverse transcriptase PCR, RNA interference microarray analysis and trans well were performed on human NSCLC tissues and cell lines. Thirty-one paired NSCLC tissues and adjacent noncancerous lung tissues were collected.
Results:
Figure 1Figure 2CD73 was upregulated in 52.38% of the lung tumor tissues and its expression was significantly related to histology and differentiation (P < 0.05). Reduced CD73 expression suppressed NSCLC cell growth in vitro. miR-30a-5p was significantly downregulated in 4 paired lung cancer tissues(with > 2.0-fold change and FDR < 0.05) and was validated in the 38 independent paired tissues (63.16%, P < 0.05), CD73 was a novel target of miR-30a-5p predicted by TargetScan, miRanda, PicTar, MirTarget2, PITA and detected by luciferase report assay, then ectopic miR-30a-5p expression in cancer cells reduced CD73 expression.
Conclusion:
CD73 play a very important role in NSCLC, and regulated by miR-30a-5p. CD73 may provide a potential target for diagnosis and treatment for lung cancer.
