Author of

• 13580

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  P1.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 233)

  • Event: WCLC 2015
  • Type: Poster
  • Track: Biology, Pathology, and Molecular Testing
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/07/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)

  • 13650

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    P1.04-070 - Silencing of Rac3 Inhibits Proliferation and Induces Apoptosis of Human Lung Cancer Cells (ID 434)

    09:30 - 09:30  |  Author(s): G. Wang
    • Abstract

    Background:
    Rac3 is a member of the Rac family of small guanosine triphosphatases (GTPases), regulates a variety of cell functions, including the organization of the cytoskeleton, cell migration, and invasion. The overexpression of Rac3 has been reported in several human cancers. However, the role of Rac3 in Lung cancer (LC) has not been determined yet. The purpose of this study is to investigate the effect of silence on Rac3 expression in human LC cells and the consequence of cell survival.
    
    Methods:
    Lentivirus small hairpin RNA (shRNA) interference techniques were utilized to knock down Rac3 gene. Gene and protein expression was quantified by quantitative Real-time polymerase chain reaction (qRT-PCR) and Western Blot. LC cell apoptosis was examined by Annexin V-APC /propidium iodide staining.
    
    Results:
    Efficient silence of of Rac3 strongly inhibited A549 cells proliferation and colony formation ability, and significantly decreased tumor growth. Moreover, flow cytometry analysis showed that knockdown of Rac3 led to G2/M phase cell cycle arrest as well as an excess accumulation of cells in the G1and S phase.
    
    Conclusion:
    Thus, functional analysis using shRNAs reveals a critical role for Rac3 in the tumor growth of LC cells. shRNAs silencing of Rac3 could provide an effective strategy to treat LC.