Author of

• 13580

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  P1.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 233)

  • Event: WCLC 2015
  • Type: Poster
  • Track: Biology, Pathology, and Molecular Testing
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/07/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)

  • 13637

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    P1.04-057 - Wnt Signaling Regulates Cancer Stem Cell in Lung Cancer SPC-A1 Cells (ID 633)

    09:30 - 09:30  |  Author(s): J. Sun
    • Abstract

    Background:
    Wnt signaling is invovled in driving cancer stem cells (CSCs) activity in a variety of cancers. The aim of this study was to explore the role of Wnt signaling in the lung cancer stem cells (LCSCs).
    
    Methods:
    Sphere culture was processed by treating human lung adenocarcinoma cell line SPC-A1 with IGF, EGF and FGF-10 to obtain the LCSCs. After confirming the stemness by immunoflurescence, functional genome screening and RT-PCR were employed to perform pathway analysis. The relationship between the identified signaling pathway and stemness gene expression was explored by agnoist/antagonist assay. Moreover, the effects on sphere formation, cell viability and colony formation by different signaling molecule inhibitors were also analyzed.
    
    Results:
    The results showed that LCSCs were successfully generated and the phenotype characterization was confirmed by expressing pluripotent stem cell markers Nanog and Oct 4, and lung distal epithelial markers CCSP and SP-C. The involvement of Wnt pathway in LCSCs was confirmed by functional genome screening and verified by RT-PCR. The expression of Wnt signaling components were related with the expression of the Nanog and Oct 4. Anti-cancer effects could be exerted by using different signaling molecule inhibitors targeting Wnt signaling pathway.
    
    Conclusion:
    In conclusion, Wnt signaling pathway is involved in the stemness regulation of LCSCs and could be considered as a potential therapeutic target in the lung adenocarcinoma.