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W. Dyszkiewicz
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O29 - Cancer Control & Epidemiology IV (ID 132)
- Event: WCLC 2013
- Type: Oral Abstract Session
- Track: Prevention & Epidemiology
- Presentations: 1
- Moderators:P. Zimmerman, N. Kurimoto
- Coordinates: 10/30/2013, 10:30 - 12:00, Bayside 103, Level 1
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O29.04 - A new medical device for in vivo isolation of circulating tumor cells in non-small cell lung cancer (NSCLC) patients and immunofluorescence identification of ALK (ID 2659)
11:05 - 11:15 | Author(s): W. Dyszkiewicz
- Abstract
- Presentation
Background
Circulating tumor cells (CTCs) in the bloodstream of lung cancer patients provide a source for early detection, prognosis, and therapy monitoring. CTCs are currently mostly isolated in vitro from small volumes of patient blood samples. In order to increase the sensitivity of the CTC detection in the peripheral blood GILUPI has developed a novel in vivo method, the CellCollector, which enables the capture of CTCs from the patient´s blood stream with a higher sensitivity and efficacy than existing methods. Enumeration and characterization of those CTCs will serve to improve and monitor clinical cancer early detection and treatment. The aim of this study was to assess the Detektor CANCER01 (DC01) for in vivo isolation of CTCs directly from the blood of NSCLC patients, and to compare it to the CellSearch® method.Methods
The device was inserted in a cubital vein through a standard cannula for thirty minutes. The interaction of target CTCs with the DC01 was mediated by an antibody directed against the epithelial cell adhesion molecule (EpCAM). To confirm the CTCs binding to the wire, the immunohistochemical staining against EpCAM and/ or Cytokeratins as well as CD45 for negative cell selection and nuclei counterstaining was performed. There were enumeration data available for 34 stage I-IIIB NSCLC patients and 8 non cancer patients. For 34 patients, samples were also tested with the CellSearch® method.Results
In this study, we successfully isolated EpCAM-positive tumor cells in the peripheral blood originating from NSCLC patients. We obtained in vivo CTC detection rate of 94% in 32 of 34 NSCLC patients with a median (range) of 13 (0-300) CTCs. In 2 of 34 samples (5.8 %), CTCs were detected by the CellSearch® method. In all matched pairs, the DC01detected the same number or more CTCs compared to the CellSearch® method. The sensitivity was similar for early and late stage NSCLC patients. In the non-cancer patients, no CTCs were detected (100 % specificity). ALK gene rearrangements in NSCLC patients are an indication for targeted therapy with crizotinib. Therefore the staining for CTCs on the CellCollector was enhanced to identify the anaplastic lymphoma kinase (ALK). It could be identified by capturing cells and immunohistochemical staining.Conclusion
Due to a detection rate of over 90% this new device might overcome present limitations in the enrichment of CTCs. This proof of concept study may have important clinical implications, as the implementation of the device into clinical practice may improve early detection, prognosis and therapy monitoring of NSCLC patients. The performed IHC for ALK expression on samples using a novel combination of a new ALK antibody with the high detection rate of the CellCollector offers an alternative to FISH or IHC on tumor tissue. This new technology also allows, as the captured tumor cells are ready using immunofluorescence approaches or qPCR, the possibility of establishing more personalized treatment regiments.Only Members that have purchased this event or have registered via an access code will be able to view this content. To view this presentation, please login, select "Add to Cart" and proceed to checkout. If you would like to become a member of IASLC, please click here.
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P3.20 - Poster Session 3 - Early Detection and Screening (ID 174)
- Event: WCLC 2013
- Type: Poster Session
- Track: Imaging, Staging & Screening
- Presentations: 1
- Moderators:
- Coordinates: 10/30/2013, 09:30 - 16:30, Exhibit Hall, Ground Level
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P3.20-002 - First low dose chest CT lung cancer screening programs in Poland - overall results (ID 928)
09:30 - 09:30 | Author(s): W. Dyszkiewicz
- Abstract
Background
To present results of the first four lung cancer (LC) screening programs based on low dose chest CT (LDCT) conducted in Poland.Methods
Four LC screening programs were conducted in different Polish cities (Gdansk, Poznan, Szczecin, Warsaw) between 2008 and 2011. Algorithms used for patient`s selection for further diagnostics were based on IELCAP protocols with local modifications. The enrolled patients were ages 50-75, being either active smokers or with history of >20 pack years. The following data were analyzed: number of LC detected and resected in both protocols, and percentage of stage I cancers detected.Results
34810 patients were screened in accordance with protocols. Results are presented in table I.
The overall detection rate was 1 cancer per 102 CTs and varied from 1/125 to 1/90 (1.05%, 1.03%, 1.0%, 0.86%, 0.98% respectively). The majority of cancers were diagnosed in stage I (64%, 55%, 70%, 69% 64.5% respectively), while this parameter does not exceed 30% in overall population treated by thoracic surgery in Poland. The differences between centers regarding both detection rate and stage I percentage were not significant (p=0.167 and p=0.599 respectively). However, if one compares the biggest center alone (Szczecin) vs all other ones together, the detection rate in Szczecin is significantly lower (p=0.0278).Parameter G P W Sz All N[o ]of patients 8693 9357 1740 15020 34810 LC detected 91 96 17 120 324 LC stage I 58 52 12 83 205 Conclusion
The detection rate of all LC as well as stage I LC in the Polish LDCT screening programs is high and comparable in different centers.
