Author of

• 12418

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  P3.05 - Poster Session 3 - Preclinical Models of Therapeutics/Imaging (ID 159)

  • Event: WCLC 2013
  • Type: Poster Session
  • Track: Biology
  • Presentations: 1
  • Moderators:
  • Coordinates: 10/30/2013, 09:30 - 16:30, Exhibit Hall, Ground Level

  • 12430

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    P3.05-013 - Effect of PsA derivatives on DNA methyltransferase (DNMT) inhibition and radiosensitization in A549 lung cancer cell line (ID 2214)

    09:30 - 09:30  |  Author(s): E.S. Ma
    • Abstract

    Background
    Psammplin A (PsA), a novel DNA methyltransferase (DNMT) inhibitor and histone deacetylase (HDAC) inhibitor, was reported to induce radiosensitivity in lung cancer cell line. Concerning in vivo tissue distribution characteristics, PsA was found to be highly distributed to lung tissues. However, it appeared to be unstable in biological matrices. Here we report the DNMT inhibitory effect of PsA derivatives and their potential for radiation sensitization in lung cancer cell line.

    Methods
    A549 lung cancer cell line was used in verification of DNMT inhibitory effect with cultured cell DNA extraction kit. A549 cell line was exposed to radiation with or without a total of 9 PsA derivatives for 18 hours prior to radiation, after which cell survival was evaluated via clonogenic assays.

    Results
    These 9 PsA derivatives all showed DNMT inhibitory effect and inhibited cell proliferation at the ranges of IC50 30–120μM. Furthermore, radiation clonogenic assays revealed that these compound shows radiosensitizing properties in A549 lung cancer cell line.

    Conclusion
    This preliminary data support the further investigation of these derivatives for use as radiation sensitizing agents with potential for clinical application.