Author of

• 10962

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  P1.20 - Poster Session 1 - Early Detection and Screening (ID 172)

  • Event: WCLC 2013
  • Type: Poster Session
  • Track: Imaging, Staging & Screening
  • Presentations: 1
  • Moderators:
  • Coordinates: 10/28/2013, 09:30 - 16:30, Exhibit Hall, Ground Level

  • 10971

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    P1.20-009 - New potential marker for the diagnosis of lung cancer: Hyaluronan. (ID 3087)

    09:30 - 09:30  |  Author(s): M.P. Rangel
    • Abstract

    Background
    HA concentration is elevated in several cancers, but there is no data regarding its concentration in the sputum of lung cancer patients. In this study, we examined the HA concentrations in tissue and sputum samples and its impact on the screening and diagnosis of lung cancer patients.

    Methods
    Hyaluronic Acid (HA) was examined in lung cancer tissue of 14 patients through immunohistochemistry using a HA-probe. The analysis was performed using ImageProPlus 7.0. The HA concentration in sputum samples of 90 lung cancer patients, 25 COPD patients and 15 healthy controls was also analyzed. All the patients and healthy controls selected underwent a sputum induction. Sputum samples were incubated with urea 7M at 60[o]C and afterwards incubated with a proteolytic enzyme. The levels of HA were measured by a noncompetitive ELISA-like fluorometric assay.

    Results
    It was observed a different expression pattern of HA in squamous cell carcinomas vs. adenocarcinomas specimens (p<0.05). In sputum, a significant different concentration pattern of HA was found among lung cancer, COPD and healthy individuals (p<0.001; Fig1A). Equally significant was the difference between HA in the sputum from lung cancer and healthy volunteers (p<0.001), as well as lung cancer and COPD patients (p=0.002). ROC curve between lung cancer and healthy volunteers furnished an area of 0.821 (0.727–0.915). Assuming a cut off value of 31,44ng/mg, the specificity was 100% and the sensitivity was 51% (Fig1B). ROC curve to distinguish COPD patients from lung cancer patients showed an area of 0.698 (0.600-0.797) and the cut off value of 48.3ng/mg presented 100% of specificity and 33% of sensitivity (Fig1C). Figure 1

    Conclusion
    The results presented suggest a promising role of HA in the developing and progression of lung cancer and its concentration in the sputum as a novel diagnostic marker for differentiating normal from lung cancer patients.