Author of

• 10525

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  P1.02 - Poster Session 1 - Novel Cancer Genes and Pathways (ID 144)

  • Event: WCLC 2013
  • Type: Poster Session
  • Track: Biology
  • Presentations: 1
  • Moderators:
  • Coordinates: 10/28/2013, 09:30 - 16:30, Exhibit Hall, Ground Level

  • 10546

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    P1.02-021 - Overexpression of αB-Crystallin predicts poor prognosis in Non-small-cell lung cancer (ID 1264)

    09:30 - 09:30  |  Author(s): J. Gu
    • Abstract

    Background
    αB-Crystallin has been shown to correlate with the invasion of several tumors. In this study we investigate the role and mechanism of αB-Crystallin in non-small cell lung cancer (NSCLC).

    Methods
    Twelve cases of NSCLC and matched nontumorous samples were used to analyze αB-Crystallin expression at the level of protein. Then, we up- and down-regulated the expression of αB-Crystallin in NSCLC cells with specific vshRNA and αB-Crystallin cDNA , and assessed the role of αB-Crystallin in the proliferation, invasion of NSCLC cell line, Furthermore, The molecular mechanism of αB-Crystallin in NSCLC cells were determined by genetic and functional screens. Finally, expression of αB-Crystallin was further examined by Immunohistochemistry (IHC) in tissue microarray (TMA) consisting of 208 cases of NSCLC, and the prognostic role of αB-Crystallin in NSCLC was evaluated by Kaplan-Meier and Cox regression analysis.

    Results
    The expression of αB-Crystallin in NSCLC tissues was much higher than those in nontumorous samples, and forced loss of αB-Crystallin expression suppressed the invasive potential of lung cancer cell lines, whereas up-regulated αB-Crystallin expression enhanced the cells invasion both in vitro and in vivo. Furthermore, we demonstrated overexpression of αB-Crystallin enhanced the invasion ability of lung cancer cells by resulting in lung cancer cells epithelial-mesenchymal transition (EMT) through ERK1/2/slug pathway. Clinically, we showed that αB-Crystallin overexpression is associated with lymph node metastasis and poor prognosis of NSCLC. Together, our findings indicate that αB-Crystallin may represent a potential therapeutic target and a novel prognostic marker of NSCLC.

    Conclusion
    Our findings indicate that αB-Crystallin may represent a potential therapeutic target and a novel prognostic marker of NSCLC.