Author of

• 10525

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  P1.02 - Poster Session 1 - Novel Cancer Genes and Pathways (ID 144)

  • Event: WCLC 2013
  • Type: Poster Session
  • Track: Biology
  • Presentations: 1
  • Moderators:
  • Coordinates: 10/28/2013, 09:30 - 16:30, Exhibit Hall, Ground Level

  • 10545

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    P1.02-020 - Evaluation Of Gene Expression Profiles For The Differential Diagnosis Of Lung Adenocarcinoma and Malignant Pleural Mesothelioma (ID 2381)

    09:30 - 09:30  |  Author(s): S. Özekinci
    • Abstract

    Background
    Squamous cell carcinoma is the most commonly diagnosed lung cancer type in Turkey; however lung adenocarcinoma diagnosis has risen among women and nonsmokers. MPM is common in Turkey because of the high asbestos exposition rates. Mostly, lung adenocarcinoma cannot be clearly differentiated from malignant pleural mesothelioma (MPM). Because of The limitations in the immunohistochemical methods there has been a growing interest in the use of gene expression profiling for diagnosis in many cancers. So we aimed to evaluate the gene expression profiles in tumor cells by using RT-PCR array, between two separate groups of lung adenocarcinoma and MPM patients.

    Methods
    Ten newly diagnosed patients with adenocarcinoma and paraffin-embedded tissues of 12 patients with MPM were included in this study without considering gender differences. Eight healthy individual were recruited as a control group. After processing the fresh samples of lung adenocarcinoma stored at -80°C for RNA isolation, cDNA synthesis and the expression of 84 genes were associated with DNA repair were analyzed with RT-PCR Array. Paraffin tissues of patients with MPM were deparaffinized and the same procedure was applied. Fold change values of gene expressions in each group are calculated in “SA Bioscience” data analysis expression page.

    Results
    ACBT, B2N, GADPH AND RPLPO genes were identified as housekeeping genes. Table 1 and 2 shows the comparisons of fold change values ​​of the gene expression differences between lung adenocarcinoma, MPM and control groups.

    Table 1: Fold changes ​​of the gene expressions of lung adenocarcinoma and MPM tumor cells comparing to control group
    Gene	Adenocarcinoma/control fold change	MPM/control fold change	p value (comparing to control group) (adenocarcinoma/MPM)
    APEX2	1,6763	6,1243	>0.05 / >0.05
    BRCA1	9,5919	20,2646	>0.05 / 0,01558
    BRCA2	4,3804	10,8169	>0.05 / 0,012071
    CCNH	2,3922	4,4773	>0.05 / 0,032102
    CDK7	3,909	15,4192	>0.05 / 0,019161
    LIG4	2,2608	13,7822	0,044834 / >0.05
    MLH1	2,5581	7,5515	>0.05 / 0,013792
    MLH3	5,9579	15,4275	>0.05 / >0.05
    MSH3	2,2494	10,2785	>0.05 / >0.05
    MSH4	6,5356	12,0767	>0.05 / >0.05
    NEIL3	14,3334	80,5092	0,015299 / >0.05
    PARP1	0,9019	2,0543	>0.05 / >0.05
    PARP2	4,7127	7,4604	0,043874 /0,009579
    PARP3	2,3613	8,2119	>0.05 / 0,049911
    PMS1	1,8236	7,3582	>0.05 / 0,039034
    RAD50	2,6223	10,2765	>0.05 / 0,03758
    RAD51	2,6223	10,2765	>0.05 / >0.05
    RAD51B	4,3683	16,7622	>0.05 / >0.05
    RAD51D	3,2769	7,0688	>0.05 / >0.05
    RAD52	2,0126	5,1099	>0.05 / >0.05
    RPA3	2,8581	7,9353	>0.05 / >0.05
    PRKDC	0,5309	2,9778	>0.05 / >0.05
    SMUG1	6,7053	18,0914	>0.05 / >0.05
    TREX1	0,5115	4,1669	>0.05 / >0.05
    UNG	7,422	26,6752	0,027669 / 0,009662
    XPA	2,0485	8,8342	>0.05 / >0.05
    XRCC2	5,6758	17,6367	>0.05 / >0.05
    XRCC4	5,9765	17,0836	>0.05 / >0.05

    Table 2: The genes that fold change values ​​were statistically significant (p <0.05) in MPM and adenocarcinoma tumor cells relative to each other
    Gene	MPM / Adenocarcinoma fold change	P
    CDK7	4.39	<0.05
    MLH1	5.32	<0.05
    TREX1	9.29	<0.05
    PRKDC	7.64	<0.05
    XPA	5.54	<0.05
    PMS1	5.19	<0.05
    UNG	4.93	<0.05
    RPA3	2.97	<0.05

    Conclusion
    We showed that adenocarcinoma and MPM tumor cells have different expression profiles of DNA repair genes. Our study suggests that TREX1, PRKDC, PMS1 genes can be significant in support of differential diagnosis between MPM and lung adenocarcinoma.