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A. Zambrano
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P3.06 - Poster Session/ Screening and Early Detection (ID 220)
- Event: WCLC 2015
- Type: Poster
- Track: Screening and Early Detection
- Presentations: 1
- Moderators:
- Coordinates: 9/09/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P3.06-012 - cfDNA in Lung Cancer Patients and People with High Risk of LC Exposed to Environmental Pollution (ID 950)
09:30 - 09:30 | Author(s): A. Zambrano
- Abstract
Background:
In Chile, Lung Cancer (LC) has the second high mortality rate/100,000 for cancer, after to stomach cancer. During 2012, in the country the LC incidence for male was 17.1 with a mortality of 17.0, while for female the incidence was a 10.2, with a mortality of 8.8. In the same period, the Antofagasta region (North of Chile) showed a rate mortality of 31.6 for both gender, with a rate of 44.2 and 17.4, for men and women, respectively. According to this point of view, early detection and screening are imperative in order to increase survive to 5 years. Non invasive biomarkers, as complementary tools, might contribute with the early diagnosis of LC. In this context, free-circulating DNA (fcDNA) levels have been described as a potential tool to detect in comparison with controls.
Methods:
Volunteers enrolled, were classified as LC free subjects (C), Pre Neoplastic Lesions (PNL) and Lung Cancer (LC), according to results of Quantitative Automatic Cytology (QAC) in sputum specimen, DR70 tumor marker, and Auto fluorescence Bronchoscopy (AFB). Free circulating DNA (fcDNA) was isolated from serum of the three groups of volunteers, quantified by qPCR and amplified. The amplified fcDNA was co-hybridized against genomic DNA from total blood, using microarray-HGC.
Results:
The LC patients showed significantly higher levels of fcDNA (average, 16,13 ng/mL) than volunteers cancer free (CF) (p < 0.01) (average 2,692 ng/mL) and with Pre neoplastic Lesions (PNL) (p < 0.001) (average 1,961 ng/mL). Additionally, four recurrent and significant deletions were detected in 2p, 7q, 11q and 17p of LC volunteers. Non significant alterations were detected in PNL. Genes located in segments with CNAs were related in immune response, xenobiotic metabolism and oxidative phosphorilation and associated to , cell proliferation, cell cycle regulation, apoptosis, differentiation and cellular adhesion and migration, all functions relevant to neoplastic progression.
Conclusion:
. In conclusion; fcDNA levels were significantly associated to LC but not to PNL related to LC, fcDNA concentration could be a non invasive and complementary tool to diagnosis of LC, molecular characteristic of fcDNA suggest that its might be used as biomarker associated to malignancy of LC, as a non invasive and complementary tool for the diagnosis of LC. Supported by INNOVA CORFO Chile: Grants 07CN13B48 and 11IDL2-10634.
