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L. Zhang
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P3.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 235)
- Event: WCLC 2015
- Type: Poster
- Track: Biology, Pathology, and Molecular Testing
- Presentations: 1
- Moderators:
- Coordinates: 9/09/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P3.04-121 - Testicular Orphan Receptor 4 (TR4) Is a Marker for Metastasis and Poor Prognosis in Non-Small Cell Lung Cancer That Drives the EMT Phenotype (ID 3120)
09:30 - 09:30 | Author(s): L. Zhang
- Abstract
Background:
Aberrant expression of Testicular orphan receptor 4 (TR4) has been shown to regulate biologic processes around solid tumors. However, it is not clear the role of TR4 in prognosis for non small cell lung cancer (NSCLC) patients and the development of NSCLC cells.
Methods:
Immunohistochemical was used to evaluate the correlation between TR4 expression and clinicolpathological characteristics in 35 paired of tumor and counterpart normal tissues and 291 cases of specimens. Knock-down assay was performed to suppress the TR4 expression level. Transwell and colony formation assays were done to investigate metastatic and proliferative abilities. Quantitative real-time PCR , western blotting and immunofluorescence staining were carried out to analyze the EMT phenotype.
Results:
Immunohistochemical evaluation of clinical samples disclosed most of the lung cancer tissues were positive for TR4, while weakly positive or negative for TR4 expression in the counterpart normal tissues. Moreover, higher levels of TR4 expression were significantly associated with higher lymph node metastases, TNM stages, tumor thrombus in vana and poor prognosis with significant difference. We observed that downregulation of TR4 with stable cell transfection significantly reduced the proliferation, invasive and metastatic abilities of NSCLC cell lines A549 and PC-9. In addition, aberrant TR4 expression could modulate the expression levels of several epithelial-to-mesenchymal transition (EMT) related markers.
Conclusion:
Collectively, our results show TR4 expression in NSCLC samples is significantly associated with poor clinicopathological features and an important role in metastatic capacity of NSCLC cells by EMT regulation.
