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J. Araujo
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P3.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 235)
- Event: WCLC 2015
- Type: Poster
- Track: Biology, Pathology, and Molecular Testing
- Presentations: 1
- Moderators:
- Coordinates: 9/09/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P3.04-112 - Repeated Observation of Immune Gene Sets Enrichment in Women with Non-Small Cell Lung Cancer (ID 768)
09:30 - 09:30 | Author(s): J. Araujo
- Abstract
Background:
There are different patterns of lung cancer (LC) characteristics between men and women. Females tend to present LC at a younger age and with more advanced stages than males; however, the prognostic is better in women. In despite of the great advances in the knowledge of the genomic landscape of lung cancer, it is not explored the molecular differences regarding to gender. Our aim was to evaluate differentially enriched gene sets between women and men.
Methods:
We evaluated 05 public databases containing gene expression values from NSCLC patients: GSE50081 (HG-U133_Plus_2; n=81 samples), GSE47115 (Illumina HumanHT-12 WG-DASL V4.0 R2; 16 samples), GSE10072 (HG-U133A; n=71 samples), GSE32863 (Illumina HumanWG-6 v3.0; 116 samples), GSE7670 (HG-U133A; n=52 samples). In each dataset, expression levels were log2 transformed and median centered. We performed the Gene Set Enrichment Analysis (GSEA) to find differences between the two genders. Each dataset was analyzed individually. Since the smoking status is the main confounding factor, datasets were divided in cohorts of smokers and non-smokers (and healthy tissues by smoking status when it was included in the dataset). Cases with unknown smoking status and former smokers were excluded from the analysis. We use the Gene ontology biological process terms to find similar enriched pathways between cohorts, 1454 gene sets named by gene ontology terms were examined. We consider a gene set enriched when at least a cohort had a p-value<0.05 and also the observation was repeated in other datasets with a p-values <0.08 (statistical trends).
Results:
The analysis showed repeated observation of immune genes enrichment in women; defense response to virus was enriched in four data sets; cytokine biosynthetic process, innate immune response, positive regulation of cytokine biosynthetic process, regulation of cytokine biosynthetic process and response to other organism were enriched in three dataset; adaptive immune response, B cell activation, cellular defense response, chemokine activity, innate immune response, interferon gamma biosynthetic process, interleukin 8 production and others were enriched in at least two data sets. On the other hand, aminoacid transport, cellular protein catabolic process, maintenance of protein localization, regulation of GTPase activity, regulation of protein polymerization, regulation of Rho GTPase cctivity and others were enriched in three datasets in men.
Conclusion:
The analysis of global gene expression showed that Immune genes sets are frequently enriched in women compared to men. Differences on enrichment pathways between men and women should be deeply explored.
