Author of

• 15382

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  P3.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 235)

  • Event: WCLC 2015
  • Type: Poster
  • Track: Biology, Pathology, and Molecular Testing
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/09/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)

  • 15441

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    P3.04-059 - Non-Small Cell Lung Cancer Mutation Analysis in Purely Caucasian Croatian Population (ID 1575)

    09:30 - 09:30  |  Author(s): F. Seiwerth
    • Abstract
    • Slides

    Background:
    Molecular profiling in lung cancer patients is crucial before starting treatment. Driver mutations in EGFR and ALK genes are targets for tyrosine kinase inhibitors. Mutation rate in domain of EGFR, ALK and KRAS genes varies between populations of lung cancer patients. The aim of the study was to analyze rates of mutation in these genes in purely Caucasian Croatian population.
    
    Methods:
    Reflex testing was performed on all non-squamous NSCLC in a period of 6 months, regardless of staging and received therapy. There were altogether 387 patients with adequate (histological and cytological) material for testing. EGFR mutations were tested first (cobas® EGFR Mutation Test, Roche), all negative were than tested for KRAS mutations (cobas® KRAS Mutation Test, Roche), and double negative samples were than tested for ALK mutation using immunohistochemistry (IHC) (clone D5F3, Ventana).
    
    Results:
    Out of 387 samples 57 had EGFR mutation (14.72%). Most common mutation was exon 19 deletion (23/57, 40.35%), while 8/57 (14.04%) had two simultaneous mutations. KRAS mutations were present in 158 samples out of 330 samples that were tested (47.88%). ALK immunohistochemistry was performed on 172 double negative samples, resulting in 12 positive cases (6.98%). When calculating with the whole cohort, we had 14.72% of EGFR positive cases, 40.82% with KRAS mutations and 3.10% of ALK IHC positive cases.
    
    Conclusion:
    gene changes rates in EGFR and ALG gene are in Croatian lung cancer patients in concordance with previously reported rates in Caucasian population, while KRAS rates are higher than previously reported. For the first time, rates of genetic changes are reported for representative sample of purely Caucasian Croatian population.
    
    

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