Author of

• 15382

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  P3.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 235)

  • Event: WCLC 2015
  • Type: Poster
  • Track: Biology, Pathology, and Molecular Testing
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/09/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)

  • 15430

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    P3.04-048 - Rare Gene Mutations in Japanese Surgically Resected Non-Small-Cell Lung Cancer Patients (ID 2715)

    09:30 - 09:30  |  Author(s): T. Yokose
    • Abstract

    Background:
    Driver gene mutations except for EGFR are rare in Japanese population. In this study, we investigated EGFR, KRAS, BRAF and PIK-3 mutations in surgically resected non-small-cell lung cancer (NSCLC).
    
    Methods:
    A total of 388 consecutive patients with NSCLC who underwent complete tumor resection in our hospital from 2006 through 2008 were studied retrospectively. Formalin-fixed, paraffin-embedded tissue sections were used to isolate DNA from carcinoma lesions. Mutational analyses of EGFR, KRAS, BRAF and PIK-3 were performed by loop-hybrid mobility shift assay, a highly sensitive polymerase chain reaction-based method.
    
    Results:
    We identified 185 EGFR mutations (47.7%), 33 KRAS mutations (8.5%), 3 BRAF mutations (0.77%), and 4 PIK-3 mutations (1.03%). In patients with BRAF mutation, all three patients were adenocarcinomas and smokers. There was no mutual mutation with EGFR and KRAS. PIK-3 mutations include 2 adenocarcinomas and 2 squamous cell carcinomas. Three of 4 patients were smoker. We found one PIK-3 and EGFR double mutation case.
    
    Conclusion:
    In Japanese surgically resected NSCLC, there are a lot of EGFR mutations, but there was little KRAS mutation. Although new molecular targeted therapy is expected, BRAF and PIK-3 mutations were very rare. Highly smoking rate in patients with KRAS and BRAF mutations was not different from past reports, but we could not find other clinical characteristics. Histopathologically, correlation between PIK-3 mutation and small cell carcinoma is attracting attention recently. In this study, histological types of cases with PIK-3 mutation were various.