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Y. Cheng
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P2.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 234)
- Event: WCLC 2015
- Type: Poster
- Track: Biology, Pathology, and Molecular Testing
- Presentations: 1
- Moderators:
- Coordinates: 9/08/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P2.04-108 - The Role of miR-30c-2* in Clinical Outcome and Drug Resistance in HPV-Infected Non-Small Cell Lung Cancer (ID 450)
09:30 - 09:30 | Author(s): Y. Cheng
- Abstract
Background:
Lung cancer is leading cause of cancer death in Taiwanese women who are mostly to be life-time never smokers. Majority of drugs and combinations are used to treat with smoking lung cancer patients, not for nonsmokers. However, the 5-year survival rate in lung cancer patients remains ~15% during the past three decades. Therefore, dissolving tumor recurrence and drug resistance is urgent needed for improving outcome in lung cancer, especially in nonsmokers. Mir-30c-2* has been considered to be tumor suppressor gene in various cancers. MiR-30c-2* levels were associated with in gemcitabine sensitivity of lung cancer cells. Down-regulation of miR-30c promotes tumor invasion via an increased in MTA1 expression.
Methods:
We examined whether miR-30c and HPV oncoprotein expression could be associated with patients’ outcome by collecting 150 lung tumors from patients with NSCLC to determine miR-30c, MTA gene expression,HPV 16/18 infection, and HPV 16/18 E6 and p53 protein expression by PCR-RFLP, nested-PCR, and immunohistochemical analysis.
Results:
Our previous reports have indicated that HPV16/18 infection may be involved in Taiwanese lung tumorigenesis. Preliminary data showed that miR-30C-2* levels were elevated 45-fold in E6-knockdown TL-1 cells as compared with parental cells with non-specific RNAi tranfection. More interestingly, MTA-1 expression was negatively correlated with miR-30C-2* in lung tumors from lung cancer patients. Expression levels of MTA-1 were positive correlated with tumor stage and nodal metastasis in tumor tissues of lung cancer patients. Our cell model studies also found that miR-30C-2* suppressed by E6 could contribute to tumor metastasis and drug resistance via an increased in MTA-1 expression.
Conclusion:
These results were showed that miR-30C-2* levels in patients’ tumor tissues could be useful to predict outcome and therapeutic response and to select useful therapy drugs for lung cancer patients, especially in patients with HPV-infection.
