Author of

• 14490

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  P2.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 234)

  • Event: WCLC 2015
  • Type: Poster
  • Track: Biology, Pathology, and Molecular Testing
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/08/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)

  • 14579

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    P2.04-089 - Detection of Epidermal Growth Factor Receptor (EGFR) Mutations in Circulating Tumor DNA During EGFR-Tyrosine Kinase Inhibitor Treatment (ID 575)

    09:30 - 09:30  |  Author(s): M. Yun
    • Abstract
    • Slides

    Background:
    Epidermal growth factor receptor (EGFR) mutations are predictive marker of EGFR-tyrosine kinase inhibitor (TKI) therapy. We compared the sensitivity of EGFR mutation detection techniques between tumor tissue and peripheral blood sample in patients with EGFR-TKI treatment.
    
    Methods:
    We collected plasma and serum sample before EGFR-TKI and after acquired resistance in 11 patients with EGFR mutations (5 cases of 19 deletion and 5 cases of L858R) from paraffin-embedded tissues using PNAClamp[TM]EGFR Mutation Detection kit. DNA extraction from plasma and serum was performed using the QIAamp MinElute virus spin kit. EGFR mutation analysis for blood was done by pyrosequencing and PANAMutyper[TM]R EGFR kit. The degree of agreement was evaluated by Cohen's kappa value.
    
    Results:
    The median EGFR-TKI duration of total 11 (7 male, 4 female) cases was 6 months (range 4-24). The sensitivity of plasma EGFR mutations were 33.3% in pyrosequencing. The sensitivities of PANAMutyper[TM]R were 72.7% in plasma and 45.5% in serum sample. The degree of agreements between tissue and blood sample were better in plasma PANAMutyper[TM]R (k=0.429, p=0.033) and serum PANAMutyper[TM]R (k=0.290, p=0.026) than plasma pyrosequencing (k=0.194, p=0.087). After the development of acquired resistance, plasma EGFR mutations were still detected in 4 cases by PANAMutyper[TM]R. One of them showed 19 deletion and T790M mutation at the same time.
    
    Conclusion:
    The sensitivity and the strength of agreement of PANAMutyper[TM]R test were better than pyrosequencing. So this technique can be useful to detect EGFR mutation in peripheral blood.
    
    

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