Author of

• 11515

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  P2.05 - Poster Session 2 - Preclinical Models of Therapeutics/Imaging (ID 158)

  • Event: WCLC 2013
  • Type: Poster Session
  • Track: Biology
  • Presentations: 1
  • Moderators:
  • Coordinates: 10/29/2013, 09:30 - 16:30, Exhibit Hall, Ground Level

  • 11531

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    P2.05-016 - Thoracoscopic Color and Fluorescence Imaging System for Sentinel Lymph Node Mapping in Porcine Lung Using Indocyanine Green-Neomannosyl Human Serum Albumin (ID 2601)

    09:30 - 09:30  |  Author(s): Y.H. Quan
    • Abstract

    Background
    This study was performed to validate a newly developed sentinel lymph node (SLN) targeting tracer, indocyanine green–neomannosyl human serum albumin (ICG:MSA), and a thoracoscopic version of the intraoperative color and fluorescence imaging system (ICFIS) for lung cancer SLN mapping.

    Methods
    5 μg/kg ICG concentrations of ICG alone or ICG:MSA were injected into rat thigh and the results were compared. The fluorescence signal-to-background ratio (SBR) of SLNs were recorded and evaluated over a 2-h period using ICFIS. In addition, a SLN biopsy by video-assisted thoracoscopic surgery (VATS) was performed using ICG:MSA in porcine lung by using thoracoscopic ICFIS.

    Results
    The newly developed ICG:MSA showed a significantly improved SBR compared to ICG alone throughout the trials. All SLNs were identified in both rat (10 SLNs in 10 rat thighs) and pig (10 SLNs in 10 porcine lungs) under in vivo conditions. All SLNs were dissected successfully using VATS with help of thoracoscopic ICFIS.

    Conclusion
    ICG:MSA is accumulated in the SLN by uptake and retention through the mannose-specific receptors on a macrophage. Thoracoscopic ICFIS successfully assisted SLN mapping despite low near-infrared (NIR) light transmission in the commercial thoracoscope. Based on the results of the thoracoscopic SLN mapping, we expect that the ICG:MSA and thoracoscopic ICFIS can be translated to clinical trials in the near future.