Author of

• 11454

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  P2.01 - Poster Session 2 - Cancer Biology (ID 145)

  • Event: WCLC 2013
  • Type: Poster Session
  • Track: Biology
  • Presentations: 1
  • Moderators:
  • Coordinates: 10/29/2013, 09:30 - 16:30, Exhibit Hall, Ground Level

  • 11466

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    P2.01-012 - Pequi fruit (Caryocar brasiliense Camb) protects against oxidative stress induced by urethane in a experimental model of lung cancer. (ID 1479)

    09:30 - 09:30  |  Author(s): M. Hage
    • Abstract

    Background
    Pequi (Caryocar brasiliense Camb) is a Brazilian fruit that has high concentrations of antioxidants, such as vitamin C and E, carotenoids, phenolic compounds and essential oils. Consumption of foods high in antioxidants, particularly carotenoids and phenolic compounds have been associated in the prevention of oxidative damage caused by reactive species (ROS), including DNA damage, and can reduce the risk of cancer, atherosclerosis and other degenerative diseases. The aim of this study was to estimate the antioxidant enzyme activity of catalase, superoxide dismutase and glutathione peroxidase and to evaluate the antioxidant activity of the pequi oil, measuring lipid peroxidation, DNA damage and nitric oxid synthases expression.

    Methods
    The study was performed in 40 male BALB/c mices: 35 animals were submitted to two doses of 1,5g/kg intraperitoneal of urethane (U=5), 10 of these mices received by gavage 15μL of pequi pulp oil (U+O), 10 animals received by gavage 15μL of ethanolic extract of pequi pulp (U+E=10) and the other 10 animals received by gavage 3μg/kg of betacarotene (U+B). 5 mices didn’t receive the urethane doses neither the gavage (C=5). After 60 days, the groups were sacrificed. The enzymatic antioxidant defense was measured by biochemical test. The antioxidant activity of pequi oil was evaluated in the lung tissues by the biochemical TBARS test (Thiobarbituric acid-reactive substances) and the DNA damage by the comet test method. Nitric Synthase expression was analyzed by imunohistochemestry.

    Results
    The lung parenchyma from the Urethane groups that received and didn’t receive gavage, showed neoplasic formations induced by the chemical carcinogenesis in contrast with control group (C). The results of the TBARS test showed a significant decreased of the lipid peroxidation in the groups that were treated by gavage (U+O)(U+E)(U+B), when compared with Urethane group (U) (p<0,05). In the same way, the image analysis of the comet assay showed a statistical significant decreased of the DNA damage cells in the groups that received treatment by gavage (U+O)(U+E)(U+B) when compared with urethane group (p<0.001). The nitric oxid synthases expression was higher in the urethane group (U), but showed a significant decrease in groups that received the gavage (p<0,05). The catalase, glutathione peroxidase and superoxide dismutase test, as well as the ratio between then, didn’t show a significant difference.

    Conclusion
    We conclude that because of its composition, high in antioxidants, pequi fruit is efficient to diminish the oxidative stress status (DNA damage and lipid peroxidation) and the nitric oxid synthases expression in chemical carcinogenesis induced by urethane. Our results also suggest that the antioxidant enzyme defense are not envolved in this process, suggesting that antioxidants present in pequi fruit may have a greater impact in lung cancer treatment.